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Figure 3: miR-15b inhibited ADP ribosylation factor-like 2 expression and decreased adenosine triphosphate levels in PC12 cells. (a) Representative Western blot of ADP ribosylation factor-like 2 after transfection with miR-15b mimic or control for 24, 48 and 72 h. β-Actin served as a loading control. (b) Statistical analysis of Western blot. miR-15b mimic led to downregulation of ADP ribosylation factor-like 2 protein levels in PC12 cells. n = 4 per group. (c) Cellular adenosine triphosphate levels in different treatment groups. 37°C, normothermia; 33°C, mild hypothermia. Data are expressed as mean ± standard error of mean *P < 0.05, **P < 0.01, by one-way analysis of variance and Tukey's test

Figure 3: miR-15b inhibited ADP ribosylation factor-like 2 expression and decreased adenosine triphosphate levels in PC12 cells. (a) Representative Western blot of ADP ribosylation factor-like 2 after transfection with miR-15b mimic or control for 24, 48 and 72 h. β-Actin served as a loading control. (b) Statistical analysis of Western blot. miR-15b mimic led to downregulation of ADP ribosylation factor-like 2 protein levels in PC12 cells. <i>n</i> = 4 per group. (c) Cellular adenosine triphosphate levels in different treatment groups. 37°C, normothermia; 33°C, mild hypothermia. Data are expressed as mean ± standard error of mean *<i>P</i> < 0.05, **<i>P</i> < 0.01, by one-way analysis of variance and Tukey's test